|
KMID : 1098420050130050270
|
|
Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 5 p.270 ~ p.275
|
|
|
Expression of a Cu-Zn Superoxide Dismutase Gene in Response to Stresses and Phytohormones in Rehmannia Glutinosa
|
|
Park Myoung-Ryoul
Ryu Sang-Soo
Yoo Nam-Hee
Yu Chang-Yeon
Yun Song-Joong
|
|
|
Abstract
|
|
|
|
Superoxide dismutases (SOD) are metalloenzymes that convert O_2^-;to;H_2O_2. Rehmannia glutinosa is highly tolerant to paraquat-induced oxidative stress. The primary objective of this study was to characterize regulation of SOD gene expression in R. glutinosa in response to oxidative stresses and hormones. A full-length putative SOD clone (RgCu-ZnSOD1) was isolated from the leaf cDNA library of R. glutinosa using an expressed sequence tag clone as a probe. RgCu-ZnSOD1 cDNA is 777 bp in length and contains an open reading frame for a polypeptide consisted of 152 amino acid residues. The deduced amino acid sequence of the clone shows highest sequence similarity to the cytosolic Cu-ZnSODs. The two to three major bands with several minor ones on the Southern blots indicate that RgCu-ZnSOD1 is a member of a small multi-gene family. RgCuZnSOD1 mRNA was constitutively expressed in the leaf, flower and root. The expression of RgCu-ZnSOD1 mRNA was increased about 20% by wounding and paraquat, but decreased over 50% by ethylene and GA_3. This result indicates that the RgCu-ZnSOD1 expression is regulated differentially by different stresses and phytohormones at the transcription level. The RgCu-ZnSOD1 sequence and information on its regulation will be useful in investigating the role of SOD in the paraquat tolerance of R. glutinosa.
|
|
|
KEYWORD
|
|
|
Cu-Zn superoxide dismutase (Cu-ZnSOD), oxidative stress, paraquat, Rehmannia glutinosa
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|